x 105 cells  (ATCC)

Journal: Imaging Neuroscience

Article Title: Detecting glioblastoma infiltration beyond conventional imaging tumour margins using MTE-NODDI

doi: 10.1162/imag_a_00472

Figure Lengend Snippet: Fig. 1. Representative coronal T2WI images and maps of MTE-NODDI NDI, fen, ODI, and DTI MD. Top panel: representative maps from a naive mouse at the third timepoint showing no discernable changes across both hemispheres. Bottom panel: maps from a G144 tumour-bearing mouse at the third timepoint revealing changes due to tumour cells infiltrating into the peritumoural margins. Tumour bulk identified on the T2WI is indicated by a dashed line on all maps.

Article Snippet: 8– 12 week- old immunocompromised mice (n = 7) (purchased from Charles River Laboratories) underwent stereotactic implantation of 1 x 105 GFP- labelled G144 cells (anteroposterior 0, mediolateral - 2.5, dorsoventral - 3).

Techniques:

Journal: Imaging Neuroscience

Article Title: Detecting glioblastoma infiltration beyond conventional imaging tumour margins using MTE-NODDI

doi: 10.1162/imag_a_00472

Figure Lengend Snippet: Fig. 5. MTE-NODDI values in the cortex correlated with GFP+ fluorescence from tumour mice. Top Panel: Representative T2WI image, fen map, MD map, and GFP+ histological image from a G144 tumour mouse. Tumour bulk as identified on the T2WI is indicated by a dashed black line. The cortical ROI used for correlation is indicated in solid yellow. Bottom Panel: Correlation of fen, NDI, and MD to GFP+ fluorescence. MTE-NODDI and MD values were extracted from a region drawn in the cortex across both hemispheres. GFP+ fluorescence is calculated as % Area under fluorescence from an equivalent cortical region drawn on immunohistological images (n=5). Pearson’s correlation coefficient was used to investigate a possible correlation (r2 values reported on respective plots).

Article Snippet: 8– 12 week- old immunocompromised mice (n = 7) (purchased from Charles River Laboratories) underwent stereotactic implantation of 1 x 105 GFP- labelled G144 cells (anteroposterior 0, mediolateral - 2.5, dorsoventral - 3).

Techniques: Fluorescence